[Eric Hofacket]

Help with understanding FISH results from bone marrow biopsy

by Eric Hofacket on Tue Dec 17, 2013 10:37 pm

Can somebody help me with understanding the results of the FISH probe I had done on my biopsy in November. My Oncologist said the results were normal but we did not have time to go over it in great detail and I am trying to understand it better. If I am reading this correctly, the results were normal (not present) for the chromosome defects tested for. But only a limited set of defects was tested for so I could have defects that were not tested for. Were the defects tested for only the high risk defects? Are there high risk defects that were not tested for? The biopsy also showed 5% monoclonal plasma cells. Overall I think this may not be that bad for being 2 years Revlimid maintenance post SCT.

DNA FISH PROBE
Comments: multiple myeloma Panel LSI ATM (11q22.3) SG/TP(53) SO, LSI RB1(13q14), LS FGFR3 (4p16) SO/IGH (14q32) SG dual color, dual fusion and LSI IgH (14q32) SG/MAF (16q23) SO dual color dual fusion Vysis, inc.
SCREENED CELLS, MOLGEN 800
IMAGED CELLS 4
FINAL RESULT
Comments: Nuc ish(ATM,TP53)x2{200},(RB1x2){200},(FGFR3,IGH)x2{200},(IGH,MAF)x2{200}
INTERPRETATION
Comments: Normal for loci ATM, TP53 and RB1. Negative for FGFR3/IGH and MAF/IGH translocations in 200 cells analyzed per probe.
COMMENT 16
Comments: Flourescence in situ hybridization (F.I.S.H) with the LSI ATM (11q22.3) SG/TP53(17p13.1) SO, LSI RB1(13q14), LSI FGFR3/IGH {t(4;14)}, and LSI IgH/MAF {t(14;16)} probes reveled 2 signals each for chromosomes 11q22.3 (ATMx2), 17p13 (TP53x2), 13q14(RB1x2), 4p16(FGFR3x2)/14q32(IGHx2), and 14q32(IGHx2)/16q23(MAFx2) in a total of 800 cells analyzed. These findings are considered a normal study for the probe panel.

[Multibilly]

Re: Help with understanding FISH results from bone marrow bi

by Multibilly on Fri Dec 20, 2013 5:54 pm

Hey Eric,

I find the summary of your FISH probes, loci to be cryptic, to say the least. While not obvious, my FISH analysis was a lot easier to decode. I honestly can't say if all the NCCN-recommended probes are included based on the way things are listed here, but I "believe" they are (remember, this is coming from a layman). However, you can find the current NCCN Version 1.2012 recommendations below. My FISH results specifically reference the NCCN-recommended panel.

http://www.medicine.wisc.edu/~williams/myeloma.pdf

In particular, see p21

[DanaH]

Re: Help with understanding FISH results from bone marrow bi

by DanaH on Fri Dec 20, 2013 8:06 pm

Hi Eric,

I found a link to the probes used in your FISH (Vysis, Inc). Most of the ones they performed are listed under the multiple myeloma category, some others can be found in other categories. Each normal chromosome comes in a pair/set, so 2 signals (1 from each) would be normal. Looks like they screened 800 cells for these abnormalities. If you click on any of those probes, it gives you a pretty detailed description of which chromes/genes they are testing.

http://www.abbottmolecular.com/products/oncology/fish/hematology-probes.html

All the best to you.
Dana

[Eric Hofacket]

Re: Help with understanding FISH results from bone marrow bi

by Eric Hofacket on Sat Dec 21, 2013 8:12 pm

Thanks all, as near as I can tell, the results are normal for everything tested using an 800 cell sample. This is really good, likely means I do not have high risk myeloma.

[StandingTall]

Re: Help with understanding FISH results from bone marrow bi

by StandingTall on Sun Dec 22, 2013 2:36 pm

Says no translocations and there isn't any data on specific cells that look like this: +1p, +11q, -13 and 14q-....which are my abnormalities from my FISH. Looks good to my unprofessional eye.