Cytogenetics FISH Report Status: Signed Out
- Lab Results:
Process: M Mitogen: none
Duration: direct Banding: FISH
Media: RPMI
Number of cells:
ATM, TP53 200
D13S319 209
CSF1R-D5S23:D5S721 200
TCF3 203
ETV6 200
CKS1B 200
IGH-CCND1 200
CMYC 200
Probe/Control Range Patient/Interpretation
17p13.1(TP53)-1copy/0-6.0% 0%/negative
13q14.3(D13S319)-1copy/0-5.5% 4.3%/negative
5q33-34,5p15.2(CSF1R-D5S23:D5S721)-3copies/0-0.6% 0%/negative
19p13.3(TCF3)-3copies/0-0.6% 1.5%/LOW POSITIVE
12p13(ETV6)-1copy/0-3.0% 0%/negative
1q21(CKS1B)-3copies/0-1.5% 0%/negative
14q32.3-11q13(IGH-CCND1)/0-0.6% 0%/negative
11q22.3(ATM)-3copies/0-0.6% 0%/negative
8q24(CMYC)-bap/0-3.0% 0%/negative
Process: 1 Mitogen: none
Duration: 24hrs Banding: FISH
Media: BMC 30min colcemid
Number of cells:
D5S23:D5S721 201
CSF1R 201
D7Z1 200
D7S486 200
D8Z2 203
D20S108 203
Probe/Control Range Patient/Interpretation
D5S23:D5S721(5p15.2):1copy/0-2.3% 0%/negative
CSF1R(5q33-q34):1copy/0-4.3% 0.5%/negative
D7Z1(7cen):1copy/0-3.7% 0%/negative
D7S486(7q31):1copy/0-4.3% 0%/negative
D8Z2(8cen):3copies/0-1.5% 0%/negative
D20S108(20q12):1copy/0-6.0% 1.5%/negative
Separated: nuc
ish(TCF3x3)[3/203],(CKS1B,CSF1R,D5S23:D5S721,CMYC,ATM,ETV6,D13S319,TP53)x2[
200],
(IGH,CCND1)x2[200]
Non-separated: nuc ish(CSF1R,D5S23:D5S721,D7Z1,D7S486,D8Z2,D20S108)x2[200]
Additional commentary that accompanied the lab results:
This is a bone marrow aspirate from a patient with multiple myeloma. FISH
analyses with probes localizing to CSF1R (5q33-q34) and D5S23:D5S721
(5p15.2), CMYC (8q24), ETV6 (12p13), D13S319 (13q14.3), TP53 (17p13.1),
TCF3 (19p13) (probes from Abbott Molecular), and CKS1B (1q21) (Kreatech)
were performed on magnetically separated CD138 positive cells. Analyses
showed normal signal numbers for the probes for CKS1B, 5p, 5q, CMYC, ATM,
ETV6, D13S319 and TP53 within the limits of these analyses. However, 1.5%
of cells had three signals for TCF3, indicating a gain of 19p. FISH
analysis with the IGH-CCND1 probes (Abbott Molecular) showed no evidence of
a fusion signal and normal signal number for both probes within the limits
of this analysis. These results are consistent with the gain of TCF3 seen
previously in this patient. The gains of 5p, 5q and 11q seen previously in
this patient were not seen in the current sample. Additional FISH analyses
with the probes localizing to 5q33-34, 5p15.2, 7q31, 7p11.1-q11.1, the
chromosome 8 centromere and 20q12 (Abbott Molecular) were performed on
non-magnetically separated cells. Results of these analyses showed normal
signal numbers for each of these probes. This indicates that the sample is
negative for monosomy 5, 5q-, monosomy 7, 7q-, trisomy 8 or 20q- within the
limits of these analyses.
