Hello,
While translating my flow cytometry results I found that CD20 has prognostic significance, especially for cyclin D1 positive, t(11:14), monosomy 13, myeloma, which I have, but my flow cytometry results don't reflect this antigen. Does this mean that is is negative, or that it wasn't included in the panel? If it is not in the panel, which I don't think it is, how common is this?
Also, I think my aberrant plasma cells are 86%. Is that the correct interpretation of the results?
T cell % Positive B cell % Positive Myeloid/Others % Positive
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CD3 CD19 3 CD45
CD5 CD38+CD138+ 88 CD34
CD3+CD4+ CD19+CD5+ CD14
CD3+CD8+ CD38+CD56+ HLA-DR
CD3+CD7+ CD138+CD56+ 86 CD33
CD2 CD19+CD11c+ CD13
CD7 CD22 CD11b
CD19+CD38+ CD117
NK cell CD19+Kappa+ TdT
CD16 CD19+Lambda+ CD10
CD56 93 CytoKappa+ 8 CD15
CD57 CytoLambda+ 91 CD64
CD3+94+ CD19+CD52+ Glycophorin
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3 posts
• Page 1 of 1
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jhorner - Name: Magpie
- Who do you know with myeloma?: Myself
- When were you/they diagnosed?: 2013
- Age at diagnosis: 49
Re: CD20 not in flow cytometry results - what's that mean?
Hello,
So CD20 is complex. Could someone perhaps comment on my flow cytometery interpretation if you know how to determine aberrant cell percentage?
J
So CD20 is complex. Could someone perhaps comment on my flow cytometery interpretation if you know how to determine aberrant cell percentage?
J
-
jhorner - Name: Magpie
- Who do you know with myeloma?: Myself
- When were you/they diagnosed?: 2013
- Age at diagnosis: 49
Re: CD20 not in flow cytometry results - what's that mean?
Hello,
For anyone that is interested, I talked to my doctor about these FISH results and, though I didn't get all my questions answered, I got some of them answered.
He said that the "flow gates" concentrate only on plasma cells and try to test surface antigens that are most commonly associated with the plasma cell types for myeloma. To quote "CD19 (which is expressed on normal plasma cells and not tumor plasma cells) and CD56 (which is expressed on tumor plasma cells but not normal plasma cells). CD20 is only expressed in 20% of patients with plasma cell disorders and not on normal plasma cells, so it is not normally gated."
My aberrant plasma cell percentage is 86%, up from 75% on my last marrow two years ago.
I'm not sure how the cytokappa and cytolambda play into this, but I was able to discern that they represent cytoplasmic monotypic light chains. How common are these surface markers in these sorts of results? My cyto markers have changed since the last marrow as well. CytoLambda increased from 83% to 91% and CytoKappa stayed the same.
Thanks,
J
For anyone that is interested, I talked to my doctor about these FISH results and, though I didn't get all my questions answered, I got some of them answered.
He said that the "flow gates" concentrate only on plasma cells and try to test surface antigens that are most commonly associated with the plasma cell types for myeloma. To quote "CD19 (which is expressed on normal plasma cells and not tumor plasma cells) and CD56 (which is expressed on tumor plasma cells but not normal plasma cells). CD20 is only expressed in 20% of patients with plasma cell disorders and not on normal plasma cells, so it is not normally gated."
My aberrant plasma cell percentage is 86%, up from 75% on my last marrow two years ago.
I'm not sure how the cytokappa and cytolambda play into this, but I was able to discern that they represent cytoplasmic monotypic light chains. How common are these surface markers in these sorts of results? My cyto markers have changed since the last marrow as well. CytoLambda increased from 83% to 91% and CytoKappa stayed the same.
Thanks,
J
-
jhorner - Name: Magpie
- Who do you know with myeloma?: Myself
- When were you/they diagnosed?: 2013
- Age at diagnosis: 49
3 posts
• Page 1 of 1