[dianaiad]

Need FISH translation

by dianaiad on Wed Mar 13, 2013 11:32 am

I was diagnosed (officially) with stage 2 multiple myeloma yesterday. While I have some idea of what all the tests I've had so far mean, I need some help with the FISH study....could someone here translate the following into 'English teacher?"

DNA FISH PROBE
Comments:
multiple myeloma Panel LSI ATM (11q22.3) SG/p53 (17p13.1) SO, LSI RB1 (13q14), LSI FGFR3 (4p16) SO/IGH (14z32) SG dual color, dual fusion and LSI IgH (14q32) SG/MAF916q23) SO dual color dual fusion

Final result:

nuc ish (ATMx4,p53x3){106/252}/(ATMx4,p53x4) {8/252}

Interpretation:

Positive for 4 signals for the ATM gene and 3 signals for the p53 gene on chromosomes 11 and 17 respectively in 106 of 252 cells (42%).
Positive for 4 signals each for the ATM and p53 loci on chromosomes 11 and 17 respectively, in 8 of 252 cells (3.2%).

Comment 16
Comments:
Fluorescence in situ hybridization (F.I.S.H.) with the LSE ATM (11q22.3) SG/p53(17p13.1) SO, LSI RB1 (13q14), LSE FGFR3/IGH {t(4;14)}, and LSI IgH/MAF {t(14;16)} probes in the panel revealed FOUR signals for the ATM gene and THREE signals for the p53 gene on the long arm of chromosome 11 and the short arm of chromosome 17 respectively, in 106 of 252 cells analyzed (42%). In addition, the analysis showed 4 signals each for the ATM and p53 genes on chromosomes 11 and 17 respectively, in 8 of 252 cells (3.2%). These findings are POSITIVE for the gain of the ATM and p53 loci on chromosomes 11 and 17 respectively. The remaining probe set revealed two signals each for chromosomes 13q14 (RB1x2), 4p16(FGFR3x2)/14q32 (IGHx2), and 14q32 (IGHx2)/16q23 (MAFx2), and are the normal patterns for these probes.
These results may reflect an underlying hyperdiploid chromosome complement which has been reported in multiple myeloma. refer also to 0691M-13.

(sigh) There is a REASON I'm an English teacher. Help?

[Dan D]

Re: Need FISH translation

by Dan D on Wed Mar 13, 2013 5:23 pm

For a multiple myeloma patient, your results are good: You are negative for all high-risk markers; you are not missing any chromosomes, like 13, nor do you have any rearrangements like 14-16 or 4-14

Instead you fall into the low (or standard) risk HYPER-dipoloid category . You have extra copies of certain odd numbered chromosomes - 11 and 17 - which is a better place to be.

[dianaiad]

Re: Need FISH translation

by dianaiad on Wed Mar 13, 2013 7:53 pm

Dan D wrote:
> For a multiple myeloma patient, your results are good: You are negative
> for all high-risk markers; you are not missing any chromosomes, like 13,
> nor do you have any rearrangements like 14-16 or 4-14
>
> Instead you fall into the low (or standard) risk HYPER-dipoloid category .
> You have extra copies of certain odd numbered chromosomes - 11 and 17 -
> which is a better place to be.

THANK you! For some reason all the other tests were a whole lot easier to figure out, but this one? I've never typed so much gobledegook in my entire life.

[Dr. Peter Voorhees]

Re: Need FISH translation

by Dr. Peter Voorhees on Thu Mar 14, 2013 7:38 am

I agree with Dan D. You have hyperdiploid myeloma. That is a good risk cytogenetic feature.
You do NOT have a high-risk IgH ranslocation (e.g. t(4;14), t(14;16), t(14;20)) nor do you have a deletion of 17p. Good news!

Best of luck to you!

Pete V.

[dianaiad]

Re: Need FISH translation

by dianaiad on Thu Mar 14, 2013 11:19 am

Thank you for the help...I love good news. This, coupled with the fact that I don't have any bone problems (no fractures, and no lytic lesions on the X-rays) I'm counting myself pretty fortunate.

[anjeb1]

Re: Need FISH translation

by anjeb1 on Sun Mar 22, 2015 11:28 pm

I also would appreciate a translation of this FISH report:
Two copies of 5p15.31, EGRI, CEP 9, 11q13(BCL1), 13q14.3, 13q34, 14q32(IGH), CEP15, 17p13.1(p53), and CEP 17 were observed.

SPECIAL INFO
ISCN RESULT: nuc ish.
(D5S630/D5S2064,EGRI)x2(192/200),
(D9Z1,D15Z4)x2(181/200) (CCND1,IGH)x2(200)
D13S319, 13q34)x2(186/200), (TP53, CEP17)x2(187/200)

Help please!